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. 2012 Sep 18;287(45):37732–37744. doi: 10.1074/jbc.M112.389015

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — eHsp90 is elevated in more aggressive PCa cell types and is essential for cell motility. A, detection of secreted eHsp90α from the conditioned media of indicated cell pairs was assessed by ELISA. Each cell pair is lineage related, with the more metastatic derivative in black. B, scratch wound assay assessed the effects of either exogenous eHsp90 protein (3 day pre-treatment, 3 μg/ml) upon ARCaPE cell motility, or treatment of ARCaPM cells with NPGA. C, similar treatment of epithelial P69 cells with eHsp90, and NPGA treatment of the metastatic counterpart M12. D, effect of shLRP1 upon eHsp90-mediated cell motility in ARCaPE. UT refers to untreated vehicle control. Asterisks (*) indicate significance of p value ≤0.05.