eHsp90 induces molecular and morphological changes consistent with an epithelial to mesenchymal transition.
A, ARCaPE cells treated for the indicated times (1, 3, or 5 day (d)) with exogenous Hsp90 protein, and immunoblot analysis of epithelial E-cadherin (E-cad) and mesenchymal proteins N-cadherin (N-cad) and Twist. Phase-contrast images of cell morphology. B, effects of NPGA treatment of ARCaPM upon E- and N-cadherin expression and corresponding cell morphology. C, analysis of eHsp90 treatment of P69 nontumorigenic cells as in A. D, analysis of NPGA treatment of M12 as in B.