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. 2012 Sep 18;287(45):37732–37744. doi: 10.1074/jbc.M112.389015

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — eHsp90 induces molecular and morphological changes consistent with an epithelial to mesenchymal transition. A, ARCaPE cells treated for the indicated times (1, 3, or 5 day (d)) with exogenous Hsp90 protein, and immunoblot analysis of epithelial E-cadherin (E-cad) and mesenchymal proteins N-cadherin (N-cad) and Twist. Phase-contrast images of cell morphology. B, effects of NPGA treatment of ARCaPM upon E- and N-cadherin expression and corresponding cell morphology. C, analysis of eHsp90 treatment of P69 nontumorigenic cells as in A. D, analysis of NPGA treatment of M12 as in B.