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. 2012 Sep 18;287(45):37732–37744. doi: 10.1074/jbc.M112.389015

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — eHsp90 modulates the expression of multiple genes associated with EMT activation. A, a focused EMT qRT-PCR array was utilized to assess EMT-regulated genes modulated by eHsp90 in ARCaPE cells. Samples for array data were derived from two identical biological replicate experiments. B, transcript expression of E-cadherin and the indicated EMT transcriptional effectors from the array were validated by qRT-PCR in untreated (UT) ARCaPE or cells treated with eHsp90 protein for 1, 3, or 5 days (upper panel), whereas a similar analysis was performed for control or ARCaPE-eHsp90 genetically modified cells (lower panel). C, increased expression of proteolytic MMP transcripts was also validated from both protein-treated (upper panel) and ARCaPE-eHsp90-modified cells (lower panel). Quantitative PCR levels were normalized to GAPDH expression. UT refers to untreated vehicle control. Asterisks (*) indicate significance of p value ≤0.05.