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Characterization of enzymatically produced 13C-labeled BV and BR mass spectrometry standards. 13C-Labeled BV IXα and BR IXα produced in E. coli by overexpression of SynHO1 or SynHO1 plus rat BVR turned bacteria the expected green or orange color due to accumulation of each tetrapyrrole (A). The DMSO-extracted tetrapyrroles had nearly identical UV-visible absorbance spectra (B) and reverse-phase C18 HPLC retention times (C) as commercial BV IXα and BR IXα standards.
