FIGURE 2.

Regulatory domains of PKCδ are necessary but not sufficient for PDBu-induced translocation to and retention at mitochondria. A–C and E, COS-7 cells co-transfected with the indicated CFP and YFP constructs were monitored for their FRET ratio responses to stimulation with 200 nm PDBu. D, COS-7 cells transfected with the indicated PKCδ-RFP constructs were lysed and immunoblotted with phospho-specific antibodies against the activation loop (pAL), turn motif (pTM), and hydrophobic motif (pHM) of PKC and with the DsRed antibody for expression of the RFP-tagged constructs.