FIGURE 7.

Mitochondrial membrane potential modulates the retention of PKCδ at mitochondria. A, COS-7 cells co-transfected with either Mito-CFP or Golgi-CFP and PKCδ-YFP were pretreated with 500 nm of the mitochondrial uncoupler FCCP for 15 min at room temperature and compared with unpretreated controls in their FRET ratio responses to 200 nm PDBu stimulation. B, COS-7 cells co-transfected with Mito-δCKAR and PKCδ-RFP were pretreated with 500 nm FCCP for 30 min at 37 °C and compared with the unpretreated control in their FRET ratio responses to 200 nm PDBu stimulation.