FIGURE 8.

PIAS1 attenuates the association between PGC-1 and LXRs. A, PIAS1 had no preference for interacting with PGC-1β and LXRβ. B, PIAS1 decreased the association of LXRβ with PGC-1β but not with SRC-3. C, PIAS1 affected the interaction of LXRβ with both PGC-1β and PGC-1α. D, PIAS1 attenuated the association of PGC-1β with LXRβ but not with TRβ1. 293T cells were co-transfected for 48 h with the indicated plasmids. Cell lysates were immunoprecipitated (IP) with the tag antibody as indicated, and immunoblotting was performed using anti-SRC-3 (B) or tag antibodies. Representative results are shown from at least two independent experiments.