FIGURE 4.

The effect of glycerol on Aβ fibrilization. 30 μm Aβ, in 165 μm ThT and PBS, was incubated in absence or presence of 0.18 to 12% glycerol under non-agitating (A) or agitating conditions (B). Changes in ThT fluorescence were monitored (A and B) with the lag phase of fibrilization (C), the elongation rate (D) and plateau height (E) depicted. **, p < 0.03 and *, p < 0.05 when compared with Aβ without crowding agents. a.u.: arbitrary units. The mean of at least three independent assays is shown. Error bars represent ± S.E.