FIGURE 1.

Effect of resveratrol on IL-1β-induced mitochondrial changes and apoptosis in human tenocytes in monolayer cultures. A, shown is a morphological evaluation of human tenocytes in monolayer culture by transmission electron microscopy. Tenocytes were either left untreated (a–c) or were treated with IL-1β (10 ng/ml) for 24, 48, and 72 h (d–f) or pretreated with resveratrol (5 μm) for 4 h and then stimulated with IL-1β (10 ng/ml) for 24, 48, and 72 h (g–i). B, to quantify apoptosis and MC in these cell cultures, 100 cells from 20 microscopic fields were counted. The examination was performed in triplicate, and the results are provided as the mean values with S.D. from three independent experiments. ×5000; bar = 1μm. Values were compared with the control, and statistically significant values with p < 0.05 were designated by an asterisk (*).