FIGURE 14.

Effects of resveratrol and PI3K-inhibitor wortmannin on IL-1β-induced suppression of Sirt-1/SCXA interaction in human tenocytes. Serum-starved human tenocytes in monolayer culture were either treated with IL-1β alone for 0, 5, 10, 20, 40, or 60 min or pretreated with resveratrol (5 μm) or wortmannin (10 nm) for 1 h and then co-treated with IL-1β for 0, 5, 10, 20, 40, or 60 min. Whole cell lysates were immunoprecipitated with an antibody against Sirt-1 and then immunoblotted with an antibody against SCXA or vice versa. The same immunoprecipitates were then reblotted with SCXA or Sirt-1 antibody. Sirt-1 control peptide (Co Pep) was used as a control for antibody specificity.