FIGURE 8.

Effects of resveratrol and c-Src, PI3K, and Akt inhibitors on IL-1β-induced c-Src, PI3K, and Akt activation in human tenocytes. Monolayer cultured serum-starved human tenocytes were either left untreated or treated with 10 ng/ml IL-1β alone for 1 h or pretreated with different concentrations of resveratrol (0.1, 1, 5, 10 μm) for 4 h or inhibitors of c-Src (PP1, 1 μm), PI3K (wortmannin (W), 10 nm) or Akt (SH-5, 1 μm) for 1 h and then co-treated with 10 ng/ml IL-1β for 1 h. Cell lysates were then analyzed by immunoblotting using antibodies raised against c-Src, PI3K, phosphospecific-Akt, and β-actin.