FIGURE 2.

A second site with a similar cis-sequence is also affected in the mef8 mutants. The upper part shows an alignment of the nad5-676 target site with the nad6-95 site with a similar cis-element in the region −25 to +5 relative to the edited C nucleotide (bold C at nucleotide 0). Nucleotides derived from other editing events are given as bold U, nucleotides identical between the two sequences are framed. The target editing site is labeled 0. The lower part shows a cDNA sequence analysis of the second site nad6-95, at which editing is reduced to approximately 80% in the mef8-1 and to 50% in the mef8-2 mutant from the nearly 100% editing observed in wild type plants.