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Function of neuraminidase during capacitation. A, zona pellucida (ZP) binding by uncapacitated sperm (U), capacitated sperm (C), and sperm capacitated in the presence of the sialidase inhibitor DANA (C+I). B, Western blot for phospho-ERK of mouse sperm. C, FACS histogram of mouse sperm stained for PY20. D, Western blot for phospho-ERK and PY20 of human sperm (C+Sia) capacitated in the presence of free Neu5Ac. **, p ≤ 0.01 versus the control.
