FIGURE 2.

Mutations at Trp-102β and Trp-106β that reduce steric bulk increase FTase reactivity with dns-GCVWS. a, shown is the k_cat/K_m value for prenylation of dns-GCVGS, dns-GCVLS, and dns-GCVWS peptides catalyzed by WT (solid bar), W102V (checkered bar), and W102A FTase (dotted bar). b, shown is the k_cat/K_m value for prenylation of dns-GCVGS, dns-GCVLS, and dns-GCVWS peptides catalyzed by WT (solid bar), W106V (checkered bar), and W106A FTase (dotted bar). The measured k_cat/K_m values are as follows: WT FTase, 3500 ± 300 m⁻¹ s⁻¹ (dns-GCVGS), 170,000 ± 40,000 m⁻¹ s⁻¹ (dns-GCVLS), and 5,000 ± 1,000 m⁻¹ s⁻¹ (dns-GCVWS); W102V FTase, 2,390 ± 210 m⁻¹ s⁻¹ (dns-GCVGS), 52,000 ± 19,000 m⁻¹ s⁻¹ (dns-GCVLS), and 14,000 ± 3,600 m⁻¹ s⁻¹ (dns-GCVWS); W102A FTase, 5,000 ± 350 m⁻¹ s⁻¹ (dns-GCVGS), 74,000 ± 5,400 m⁻¹ s⁻¹ (dns-GCVLS), and 130,000 ± 21,000 m⁻¹ s⁻¹ (dns-GCVWS); W106V FTase, 2,000 ± 160 m⁻¹ s⁻¹ (dns-GCVGS), 34,000 ± 770 m⁻¹ s⁻¹ (dns-GCVLS), and 22,000 ± 2,200 m⁻¹ s⁻¹ (dns-GCVWS); W106A FTase, 4,700 ± 250 m⁻¹ s⁻¹ (dns-GCVGS), 70,000 ± 500 m⁻¹ s⁻¹ (dns-GCVLS), and 87,000 ± 11,000 m⁻¹ s⁻¹ (dns-GCVWS).