FIGURE 5.

Evaluation of the reactivity of WT FTase and FTase variants with target peptides. Initial velocities were measured for WT FTase and selected FTase variants in the cell lysate-based fluorescence assay at four peptide concentrations, as described under “Experimental Procedures.” a, initial velocity for reaction with dns-GCVDS is shown; squares, WT FTase; circles, W102R/W106L variant; triangles, W102L/W106L variant; inverted triangles, W106L/W106K variant. B, initial velocity for reaction with dns-GCVKS is shown; squares, WT FTase; circles, W102F/W106E variant; inverted triangles, W106L/W106E variant.