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. 2012 Sep 18;287(45):38135–38149. doi: 10.1074/jbc.M112.412577

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — WbdA^O8 contains three separable domains. The results show mutant complementation experiments with CWG1104 (ΔwbdA^O8) expressing WbdA^O8-D1+D2 (pWQ595), WbdA^O8-D2+D3 (pWQ596), WbdA^O8-D1+D3 (pWQ593 + pWQ594), or WbdA^O8-D1+D2+D3 (pWQ592 + pWQ596 or pWQ594 + pWQ595). Top, silver-stained SDS-polyacrylamide gel of LPS samples from whole-cell lysates; bottom, the corresponding Western immunoblot using O8-specific antiserum. Native O-PS biosynthesis was restored only when all three WbdA^O8 domains were present.