TABLE 2.
Data collection parameters | |
X-ray source | Australian Synchrotron |
Wavelength (Å) | 1.127 |
q range (Å−1) | 0.004–0.25 |
Sample flow rate (4 μl s−1) | 4 |
Exposure time/frame (s) | 1 |
Concentration range (mg ml−1) | 0.25–2.0 |
Structural parameters | |
I(0) (cm−1) (from Guinier plot) | |
2 mg ml−1 | 0.108 ± 0.0001 |
1 mg ml−1 | 0.051 ± 0.0001 |
0.5 mg ml−1 | 0.025 ± 0.0001 |
0.25 mg ml−1 | 0.012 ± 0.0001 |
Rg (Å) (from Guinier plot) | |
2 mg ml−1 | 58.2 ± 0.4 |
1 mg ml−1 | 57.6 ± 0.7 |
0.5 mg ml−1 | 56.9 ± 1.2 |
0.25 mg ml−1 | 57.3 ± 1.7 |
I(0) (cm−1) (from P(r))a | 0.112 ± 0.001 |
Rg (Å) (from P(r))a | 63.1 ± 0.4 |
Dmax (Å)a | 215 |
Molecular mass Mr (from I(0))a | 74,150 |
Calculated monomeric mass Mr from sequence | 63,184 |
Normalized spatial discrepancy of averaged dummy atom modelsa | 0.62 ± 0.07 |
a Reported for 2 mg ml−1 measurement, which was used for model building.