FIGURE 1.

Purified proteins. A, nonreducing SDS-polyacrylamide gel stained with GelCode blue of recombinant (1) fXI-CD/PK-HC), (2) fXI-WT, (3) fXI/PKA3, and (4) fXI/PKA2-Ser¹⁴⁰. FXI-CD/PK-HC is an 80-kDa monomer, whereas other fXI species are 160-kDa dimers. B, purified fXIa heavy chain (HC) and catalytic domain (CD) isolated from fXI-Ser³⁶²/Ser⁴⁸² are compared with fXIa-WT (XIa) on nonreducing (left) and reducing (right) SDS-polyacrylamide gels. C, fIX and fIXα on a nonreducing SDS-polyacrylamide gel. Note that fIXα migrates more rapidly than fIX, despite having the same molecular mass. Positions of molecular mass standards are indicated on the left of each panel in kilodaltons.