FIGURE 6.
Measurement of ROS generation and TrxR activity in HeLa cells exposed to ATG and ebselen. A, HeLa cells were treated with the indicated concentrations of ATG or ebselen or ATG plus ebselen for 30 min. After washing with PBS and treatment with 10 μm carboxy-H2DCFDA for 30 min, cells were washed again to remove excess H2DCFDA. Fluorescence was analyzed as described under “Experimental Procedures.” Error bars show mean ± S.D. n ≥ 3. **, p < 0.01, Student's t test, treated cells versus control. B, HeLa cells were treated with different concentrations of ATG or ebselen or ATG plus ebselen for 24 h. After treatment, TrxR activities were determined with a fluorescent insulin assay. Error bars show mean ± S.D. n = 2. **, p < 0.01, Student's t test, treated cells versus control.