Figure 5.

Hyphae growing away from a membrane after having penetrated a pore (magnification ×27). Zoospores were induced to encyst on a PET membrane. Hyphae that had successfully penetrated through pores were visualized by staining with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazollium bromide. The plane of focus in each picture (A–D) is progressively closer to the membrane surface and is slightly above the membrane in D. Arrows point to hyphae that are more visible in frames A, B, or C, indicating that they must be growing away from the membrane surface (D).