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Germination of cysts on a surface of cross-linked collagen overlying a PET membrane. Zoospores were induced to encyst on the collagen by application of an agar plug containing isoflavones to the opposite side of the membrane. Germinating hyphae penetrated the collagen layer and subsequently formed appressorial structures that are believed to be located over membrane pores. The distance indicated is the distance between two hatch marks.
