FIGURE 4.

The effect of R753Q polymorphism on TLR2-elicited cytokine gene expression. A, HEK293 cells stably transfected with plasmids encoding WT or R753Q YFP-TLR2 were treated for 3 h with medium, 1 μg/ml Pam3Cys, or 5 μg/ml M. tuberculosis (Mtb) CW preparation. B, mouse WT or TLR2^−/− iBMDMs were nucleofected with pcDNA3-YFP or expression vectors encoding human WT or R753Q YFP-TLR2 variants. After recovery for 24 h, cells were treated for 3 h with medium or 1 μg/ml Pam3Cys. RNA was isolated, converted into cDNA, and subjected to real-time quantitative PCR analyses with the respective gene-specific primers. Data were processed according to the 2^−ΔΔCT method. Shown is the summary (mean ± S.D.) of three independent experiments (A) and a representative experiment (mean ± S.E., n = 4). *, p < 0.05. ns, not significant.