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. Author manuscript; available in PMC: 2013 Oct 24.
Published in final edited form as: J Am Chem Soc. 2012 Oct 10;134(42):17582–17591. doi: 10.1021/ja3064292

Figure 3.

Figure 3

Real-time observation of isoelectric point photoswitching in avGFP. (A–C) Log-transformed fluorescence micrographs corrected for cathodic pH gradient drift showing dynamic isoelectric point evolution of avGFP isoforms during isoelectric focusing over time (100 ms streamed exposures, E = 300 V cm−1). Initial and final electropherograms in linear relative fluorescence units accompany each timelapse micrograph. The three panels show typical behavior upon applying a sequence of microchannel exposure conditions - (A) Nil-to-blue light exposure. (B) 100% UV exposure (excitation 300–380 nm, emission >410 nm). (C) Blue light exposure. Delay between micrographs due to filter cube exchange is ~2 s.