Figure 3.

Aggregation of phosphoryl-Ser derivatives of htt^NTQ₃₇P₁₀K₂ peptides. (a) Sedimentation analysis of htt^NTQ₃₇P₁₀K₂ peptides in PBSA at 37°C by sedimentation analysis. WT (F17W) (●, 19 µM); S13D/S16D (●, 34 µM); S13p( ○, 21 µM); S16p (○, 20 µM); S13pS/S16pS (●, 19.5 µM). (b–r) Electron micrographs of the aggregates of different htt^NTQ₃₇P₁₀K₂ peptides isolated at different time-points. Aggregates of: WT (F17W) at 47 hrs (b) and after 1 hr (c); S13D/S16D after 100 hrs (d–g); S13pS/S16pS after 163 hrs (hd–j); S13p (kd–l) and S16p (md–n), both at 33 hrs; S13D/S16D aggregates at 1.5 hrs (od–p) and S13pS/S16pS aggregates at 2 hrs (qd–r). Scale bar = 50 nm.