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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cancer Res. 2012 Sep 10;72(21):5576–5587. doi: 10.1158/0008-5472.CAN-12-2001

Figure 3. Loss of p53 regulated miRNAs and up-regulation of miR-34 target genes in KrasLSL-G12D/+;Trp53LSL-R172H/+ tumors.

Figure 3

Total RNA was extracted from individually dissected tumors or normal lung of uninfected control mice and transcript abundance was quantified. (a) Mature miRNA levels were measured by qRT-PCR, normalized to RNU6B, and graphed relative to normal lung tissue from 3 uninfected littermates. (b) Precursor miR-34 family members were evaluated by qRT-PCR, normalized to Actin, and graphed relative to normal uninfected lung tissue. The three points above the mean for pre-miR-34a represent tumors that were heterozygous for Trp53; the two below are hemizygous. (c) miR-34 target genes, Met, Bcl-2, Cdk-4, and Myc were evaluated by qRT-PCR, normalized to Actin, and graphed relative to normal uninfected lung tissue. In all three figures black bars indicate the mean. * p-value < 0.05, ** p-value <0.01, ***p-value <0.001. p-values were determined by two-tailed t-test.