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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cancer Res. 2012 Aug 27;72(21):5600–5612. doi: 10.1158/0008-5472.CAN-11-3666

Figure 2.

Figure 2

A) Deletion mutants of the 5’-flanking region of CD24 gene. B) Deletion mutants were transiently transfected in UMUC-3 cells and the effect of deletions on hypoxia mediated promoter activity was assessed by measuring luciferase activity as explained in Methods. * Compared to PGL-3 vector transfected cells in grown in normoxia (p<0.01), # Compared to PGL-3 vector transfected cells exposed to hypoxia (p<0.01). C) Site directed mutation of HIF-1 binding site on CD24 promoter. The region CGTG between −118 and −135 on CD24 promoter was mutated to AAAA. D) Functional activity of these mutations were measured by transfecting wild type and mutated HIF-1 element followed by exposing them to normoxia, hypoxia or by co-transfecting HIF-1α transgene together. The results are representative of three independent experiments. *Compared to wild type CD24 promoter reporter transfected cells exposed to normoxia, hypoxia or after introduction of HIF-1 transgene (p<0.01)