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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cancer Res. 2012 Aug 31;72(21):5494–5504. doi: 10.1158/0008-5472.CAN-11-3993

Figure 3. IKKε regulates expression of genes involved in cellular motility and inflammation.

Figure 3

(A) Schematic of workflow for determining the ovarian cancer-specific IKKε gene signature. First, 278 genes were experimentally determined by depleting IKKε with 2 different shRNA constructs in Ovcar5 cells. In parallel, 456 genes were found to be associated with IKKε over-expression in 283 publicly available ovarian cancer gene expression profiles (GSE9899). The intersection of these two sets identified 17 genes. (B) The relative expression of these 17 genes in 283 ovarian cancer patient specimens is ranked by average expression of the 17 genes. Correlation of each gene with IKKε expression is also noted. (C) The 17-gene expression average was calculated for each of the 6 previously published subgroups of ovarian cancer (12). Log2 average was median-centered for the entire dataset. (D, E) The 17 genes were analyzed by Ingenuity Pathway Analysis. The most significantly related pathway was a cell-motility network, centered on the NF-κB complex.