Table 1.

Phage, bacterial strains, and plasmids used in this study

Strains/plasmids	Characteristics*	Source/reference
Strains
Phage strain
PaP3	P. aeruginosa phage isolated from hospital sewage	Lab collection
Bacterial strains
P. aeruginosa PA1	Clinical isolate of P. aeruginosa, serotype 6	Lab collection
E. coli DH5α	Cloning host for maintaining recombinant plasmids	Lab collection
E. coli BL21(DE3)	Expression host for recombinant protein production	Lab collection
Plasmids
pMD™18-T	T-cloning vector; AmpR	TaKaRa
pMD-cos	Derivative of pMD™18-T with a cloned 239-bp PCR product containing the cos end sequence of PaP3	This work
pET-22b(+)	C-terminal His tag fusion expression vector; AmpR	Novagen
pQE-31	N-terminal His tag fusion expression vector; AmpR	Qiagen
pQE31-p01	Derivative of pQE-31 containing the PaP3 terminase small subunit coding gene orf1	This work
pET22b-p03	Derivative of pET-22b(+) containing the PaP3 terminase large subunit coding gene orf3	This work

* Amp^R, ampicillin resistant