Table 1.
Phage, bacterial strains, and plasmids used in this study
| Strains/plasmids | Characteristics* | Source/reference |
|---|---|---|
| Strains | ||
| Phage strain | ||
| PaP3 | P. aeruginosa phage isolated from hospital sewage | Lab collection |
| Bacterial strains | ||
| P. aeruginosa PA1 | Clinical isolate of P. aeruginosa, serotype 6 | Lab collection |
| E. coli DH5α | Cloning host for maintaining recombinant plasmids | Lab collection |
| E. coli BL21(DE3) | Expression host for recombinant protein production | Lab collection |
| Plasmids | ||
| pMD™18-T | T-cloning vector; AmpR | TaKaRa |
| pMD-cos | Derivative of pMD™18-T with a cloned 239-bp PCR product containing the cos end sequence of PaP3 | This work |
| pET-22b(+) | C-terminal His tag fusion expression vector; AmpR | Novagen |
| pQE-31 | N-terminal His tag fusion expression vector; AmpR | Qiagen |
| pQE31-p01 | Derivative of pQE-31 containing the PaP3 terminase small subunit coding gene orf1 | This work |
| pET22b-p03 | Derivative of pET-22b(+) containing the PaP3 terminase large subunit coding gene orf3 | This work |
* AmpR, ampicillin resistant