Table 2.

Rate constants from kinetic analysis of the SPR data for C.Esp1396I binding to the three operator sites, O_M, O_L and O_R

	ka (M−1.s−1)	kd (s−1)	KD (nM)
OM	1.61 ± 0.01 × 108	0.177 ± 0.001	1.10 ± 0.01
OL	2.99 ± 0.02 × 107	0.254 ± 0.001	8.5 ± 0.1
OR	3.88 ± 0.05 × 106	0.887 ± 0.004	229 ± 4

The equilibrium dissociation constants, K_D, were in each case determined from the ratio of the off-rate (k_a) to the on-rate (k_d).