Table 2.
ka (M−1.s−1) | kd (s−1) | KD (nM) | |
---|---|---|---|
OM | 1.61 ± 0.01 × 108 | 0.177 ± 0.001 | 1.10 ± 0.01 |
OL | 2.99 ± 0.02 × 107 | 0.254 ± 0.001 | 8.5 ± 0.1 |
OR | 3.88 ± 0.05 × 106 | 0.887 ± 0.004 | 229 ± 4 |
The equilibrium dissociation constants, KD, were in each case determined from the ratio of the off-rate (ka) to the on-rate (kd).