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. 2012 Aug 24;40(20):10507–10520. doi: 10.1093/nar/gks727

Figure 2.

Figure 2.

Primer extension analysis of in vitro methylation of C2498. Arrows denote stops at the nucleotide before Cm2498. (A) Recombinant RlmM modifies C2498 of IVT based on primer extension analysis. WT: 23S rRNA purified from wild-type E. coli; IVT: in vitro transcript of 23S rRNA; RlmM: IVT incubated with RlmM enzyme. U, A, C and G correspond to DNA sequencing lanes. (B) RlmM modifies C2498 of unmodified 23S rRNA domain V alone. Other labels as in A. (C) Time course of IVT methylation. Top: stars indicate bands used for comparing band intensities between lanes. Bottom: plot of modification time course. Intensity of the C2499 position at time 0 was subtracted from each time point.