Figure 1.

Discrete DNA sites regulate hotspots. Base pair substitutions can create and ablate meiotic recombination hotspots (5,6,12,13,64). An example of high resolution analysis is shown here. The DNA sequence of a hotspot allele of fission ade6 (ade6-4099) that contains an Oligo-C motif is shown in bold. Plot displays average recombinant frequencies for that hotspot allele (dashed line) and for single base pair variants of that sequence (histogram bars). The sequences required to achieve different thresholds of hotspot activity are indicated below the graph (B = G or T or C; H = A or C or T). Reproduced from (7) © 2011 with permission from the Genetics Society of America.