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. 2012 Aug 31;40(20):10041–10052. doi: 10.1093/nar/gks768

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Evaluation of the influence of the nucleotide composition of the seeds on RNA-ensign (first column), RNAinverse (second column) and NUPACK (third column). The x- and y-axis represent, respectively, the A+G content and C+G content of the sequences. The first row shows the success rates of each method; the second row shows the probability of the target structure for the designed sequences; the third row reports the Hamming distance (i.e. number of mutations) between the seed and the designed sequence; and the last row shows the distribution of the A+G and C+G content of the designed sequences.