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. 2012 Aug 31;40(20):10124–10138. doi: 10.1093/nar/gks770

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Intragenic methylation of PolgA in reprogrammed and mtDNA divergent ESCs. Bisulphite sequencing analysis of CpG methylation in (A) CC9^mus, (B) CC9^dunni, (C) iPS^QS, (D) iPS^NGFP2 (E) NT-ES, (F) iPS^NFGPinj pluripotent stem cells. (G) The percentage CpG methylation per sequence determined by bisulphite sequencing (percentage mean ± SEM). (H) Real time PCR quantification of PolgA expression in cultured CC9^mus, CC9^spretus, CC9^dunni, iPS^QS/R26 (iPS^QS), iPS^NGFP, NT-ES, iPS^NGFPin^j pluripotent stem cells expressed relative to ESD3 cells. (J) Cumulative analysis of the relationship between DNA methylation levels (Figures 1L and 3G) and the expression of PolgA (Figures 2A and 3H) performed using Pearson correlation coefficient (R²). (K) MtDNA copies/cell in cultured CC9^mus, CC9^spretus, CC9^dunni, iPS^QS/R26, iPS^NGFP, NT-ES, iPS^NGFPinj pluripotent stem cells. Values represent mean ± SEM and significant differences between cell types are: *P < 0.05; **P < 0.01 and ***P < 0.001.