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. 2012 Aug 21;40(20):10576–10584. doi: 10.1093/nar/gks786

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Design strategy of the simplified genetic code. (A) Schematic diagram of the simplified genetic code. For the reassignment, the prevention of Trp incorporation and the addition of an adapter molecule that connects the translation pathway between the UGG codon and Ala are required. (B) The nucleotide sequences of the anticodon stem loop of the tRNA^Ala variant and the UGG codon on the mRNA. The anticodon loop of tRNA^Ala was substituted for that of tRNA^Trp. Positions 32 and 38 in the anticodon loop are numbered.