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. 2012 Aug 21;40(20):10576–10584. doi: 10.1093/nar/gks786

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The translation efficiency and accuracy of the simplified code are comparable to those of the universal code. (A) The fluorescent intensities of GFP mutants synthesized by the simplified and universal codes. Results are presented as means ± SD (n = 3). (B) Superposition of the overall crystal structures of gfpΔUGG-A110X(UGG)/Sim (yellow) and gfpΔUGG/Univ (green). The chromophore is shown by a stick representation. (C) Detailed views of the amino acid at position 110 and Phe¹¹⁴ of gfpΔUGG-A110X(UGG)/Sim or gfpΔUGG/Univ, in ball-and-stick representations. The 2F_o − F_c map is contoured at 1.0 σ and overlaid on the model. The color code is as in (B).