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. 2012 Aug 24;40(20):10521–10531. doi: 10.1093/nar/gks790

Table 2.

Catalytic parameters of Pth variants

Enzyme kcat (/s) Km (μM) relative kcat/Kma
WT 11.7 ± 0.65 4.71 ± 0.77 100
N185A 5.80 ± 0.80 13.4 ± 2.36 17.4
H188A 7.93 ± 0.53 17.1 ± 2.03 18.6
N185A-H188A 8.70 ± 0.83 26.9 ± 3.95 13

aRelative kcat/Km values are shown, given an arbitrary value of 100 with the WT enzyme.