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. 2012 Aug 30;40(20):10287–10301. doi: 10.1093/nar/gks798

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Attenuation of NONO decelerates NHEJ. (A) HeLa cells knocked-down with a scrambled siRNA (lanes 3 and 4) or a siRNA directed against NONO (lanes 5 and 6) were treated for 2 hr with NCS (500 ng/ml) and allowed to recover for either 60 or 120 min. Cells were then collected, embedded and lysed in agarose blocks and used for pulse-field gel electrophoresis. (B) A linearized, ³²P-end-labeled pBluescript was incubated for the indicated times with a nuclear extract derived from HeLa cells treated with a scrambled siRNA (lanes 2–9) or an siRNA directed against NONO (lanes 10–17). (C) Quantitation of the end joining events using a phosphorimager: The percent end joining represents the total signal intensity per lane normalized to 100% from which is substracted the % intensity of the remaining template (n = 4).