Figure 6.

NONO is recruited to DNA damage sites in a PAR-dependent manner. (A) Representative images of laser-irradiated HeLa cells expressing GFP-NONO and subjected to IF for detection of PARP-1 and PAR. (B) Representative images of the laser-irradiated cells. HeLa cells were transfected either with the GFP-tagged NONO construct or with a mutant lacking the RRM1. Then cells were either left untreated, treated with 10 µM ABT-888 1 hr before irradiation or cotransfected with mCherry-PARG prior to laser microirradiation. (C) Statistical analysis of the recruitment kinetics. At least 15 cells per condition in three independent experiments were analysed for their fluorescence intensity above the background.