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H3K76me1 is undetectable in S phase cells. (A) Indirect IFA of different cell-cycle stages in PCF expressing PCNA-TY (green) and H3K76me1 (red). DNA was stained with DAPI. Scale bar, 2 µm. (B) Quantification of cell cycle-dependent expression of PCNA-TY, H3K76me1 and H3K76me2. Three independent experiments were analysed.
