Figure 4.

DOT1A over-expression causes continuous replication of nuclear DNA. Incorporation analysis of EdU and BrdU in replicated DNA during S phase of (A) wild-type and (B) DOT1A over-expressing cells (14 h post-induction). Top panels show a schematic representation of the experimental design. Labelled cells are shown as coloured dots. Bottom panels show IFA of labelled parasites. Detection of EdU (red) is based on a ‘click reaction’, BrdU (green) was detected by an antibody. DNA was stained with DAPI (blue). Scale bars, 2 µm.