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. 2012 Aug 30;40(20):10312–10323. doi: 10.1093/nar/gks807

Figure 2.

Figure 2.

DNA binding by monomeric PALB2 and D-loop formation. (A) PALB2 or PALB2Δ1–40 bind ssDNA. Electrophoretic mobility shift assays were performed with PALB2 or PALB2Δ1–40 (10–50 nM) and 60 nt ssDNA (100 nM nucleotides) and analyzed on a 8% polyacrylamidegel. Right: quantification of the results. (B) Top: schematic of D-loop assay. D-loop reactions were performed with RAD51 alone (lane 2) or combinations of RAD51 and PALB2 or PALB2Δ1–40 (lanes 3–8). The band indicated with an asterisk corresponds to annealed tailed molecules. CCC designates Covalently Closed Circular supercoiled plasmid.