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. 2012 Aug 30;40(20):10312–10323. doi: 10.1093/nar/gks807

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Stimulation of RAD51 filament formation by monomeric PALB2. (A) Left: schematic of RPA displacement assay. Right: RPA bound to a ssDNA oligonucleotide prevents RAD51 assembly (lane 2) whereas addition of PALB2 or PALB2^Δ1–40 (15, 30 and 50 nM) stimulates RAD51 filament formation in presence of RPA (lanes 3–8). Bottom: quantification of the results. The Y-axis represents the ratio between the values obtained for RAD51 filament in the presence of RPA and PALB2 or PALB2^Δ1–40 divided by the value obtained for RAD51 filament with ATP and magnesium without RPA (set at 100%). (B) Left: RPA displacement assay in the absence of ATP and MgCl₂. Right: quantification of the results. The Y-axis represents the ratio between the values obtained for RAD51 filament in the absence of ATP/MgCl₂ with or without RPA and PALB2 or PALB2^Δ1–40 divided by the value obtained for RAD51 filament with ATP and magnesium without RPA (set at 100%).