TABLE 1.
16S rRNA-targeted oligonucleotide probes and buffer wash temperatures for the experimental conditions used
| Probe | Target | Sequence (5′ to 3′) | Temp (°C) | Reference |
|---|---|---|---|---|
| S-D-Bact-0338-a-A-18 (EUB338-I) | Eubacteria | GCTGCCTCCCGTAGGAGT | 54 | 3 |
| EUB338-II | Eubacteria | GCAGCCACCCGTAGGTGT | 54 | 10 |
| EUB338-III | Eubacteria | GCTGCCACCCGTAGGTGT | 54 | 10 |
| S-*-Bacto-1080-a-A-18 (Bacto) | Bacteroides-Prevotella-Porphyromonas group | GCACTTAAGCCGACACCT | 50 | 11 |
| S-*-Bfra-0602-a-A19 (Bfra) | Bacteroides fragilis group | GAGCCGCAAACTTTCACAA | 53 | 12 |
| S-G-Enter-1432-a-A-15 (Enter) | Enteric bacteria | CTTTTGCAACCCACT | 43 | 51 |
| S-G-Clept-1240-a-A018 (Clept) | Clostridium leptum groupa | GTTTTRTCAACGGCAGTC | 48.5 | 51 |
a
The probe also detects Desulfotomaculum species according to Probe Match (http://rdp.cme.msu.edu/docs/probe_match_doc.html).