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. 2012 Aug 30;40(20):10375–10383. doi: 10.1093/nar/gks823

Figure 2.

Figure 2.

Analysis of Prochlorococcus MED4 clones by multiplex PCR. (A) Primer pairs annealing to sites distributed along the genome every 100 kb show evidence for two partial clones (clone 1–17 in yeast strain W303-1 A, ∼675 kb in size and clone 1–V12 in yeast strain VL6-48, 583 kb in size) and one whole genome (clone 1–13, 1667 kb in size) isolated from S. cerevisiae strain W303-1 A as compared to wild-type Prochlorococcus genomic DNA (wt) multiplex patterns. (B) Presence of the cloning vector only was confirmed by screening with three amplicons specific for the vector sequence (HIS3: 550 bp, lacZ: 750 bp and TetM: 1000 bp). All three clones were positive for these markers compared to negative controls (H = H2O, wt = Prochlorococcus genomic DNA). Only the endogenous his3-11,15 allele is also amplified by the HIS3 primer pair as expected in wt W303-1 A yeast genomic DNA (Y); pmycYACTn vector only (V); 100-bp ladder, Invitrogen (L).