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. 2012 Sep 8;40(20):10240–10253. doi: 10.1093/nar/gks838

Figure 3.

Figure 3.

TRAMP-dependent exosome-mediated RNA decay controls snoRNA expression. (A) Total RNA prepared from the indicated strains was subjected to northern blot analysis using probes complementary to the indicated snoRNAs. The 5 S rRNA was used as a loading control. Normalized levels of each snoRNA relative to WT cells are indicated beneath each lane. (B) Northern blot analysis of RNA prepared from WT (lanes 1–2) and nmt1-mtr4 (lanes 3–4) strains that were grown in the absence (−) or presence (+) of thiamine. Normalized levels of each snoRNA relative to WT cells cultured in the absence of thiamine (lane 1) are indicated beneath each lane. (C) Northern blot analysis of total RNA prepared from WT (lane 1) and cid14Δ (lanes 2–4) cells that were previously transformed with the empty vector (EV; lanes 1–2) or vectors that express WT (lane 3) and catalytically inactive (lane 4; DADA) versions of Cid14. Normalized levels of each snoRNA relative to WT cells (lane 1) are indicated beneath each lane. (D) Total RNA prepared from WT (lane 1) and dis3–54 (lane 2) strains were subjected to northern blot analysis using probes complementary to the indicated snoRNAs. The 5 S rRNA was used as a loading control. Normalized levels of each snoRNA relative to WT cells are indicated beneath each lane.