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The activity of TDG catalytic domain as a function of pH. The activity of TDG catalytic domain on (a) G:5caC at 37°C and (b) G:U substrates at 4°C under single turnover condition ([ETDG] = 2.5 µM and [SDNA] = 0.25 µM) at three different pH values. The reaction on G:U substrate was measured at 4°C because the reaction was complete within 1 min at 37°C or room temperature (∼22°C) (data not shown).
