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. Author manuscript; available in PMC: 2012 Nov 3.
Published in final edited form as: Biophys Chem. 2006 Apr 6;123(1):11–19. doi: 10.1016/j.bpc.2006.03.018

Fig. 6.

Fig. 6

Comparison of concentrations of intercalator–DNA and intercalator–CHL complexes calculated based on data from two-component systems (solid lines – intercalator–CHL, dashed lines – intercalator–DNA complexes, dotted lines illustrate confidence limits in concentration estimates resulting from uncertainty of Ka, Ki and n parameter estimates), and from iterative least squares fit to absorption spectra (dots – intercalator–DNA, squares –intercalator–CHL complexes, respectively). (a) AO–DNA–CHL mixtures, concentrations of AO and DNA were 2.8×10−6 M and 3.25×10−5 Mbp, respectively, (b) DOX–DNA–CHL mixtures, concentrations of DOX and DNA were 8×10−6 M and 3×10−5 Mbp, respectively, (c) QM–DNA–CHL mixtures, concentrations of QM and DNA were 8 × 10−6 M and 4.5×10−5 Mbp, respectively.