Table 2.
Species | Priming or co-administered agent | hCG [dose or source]* | Oviposition** | References |
---|---|---|---|---|
Bufo americanus |
|
Human pregnant urine |
None |
[2] |
B. americanus |
No priming |
100 IU, Day 1 |
None |
[38] |
No priming |
400-1000 IU, Day 1 |
about 60%, Day 2 |
||
Priming Day −2 [LH 50 ug, or LHRH 50 ug, or eCG 50 IU, or hCG 50 IU] |
500 IU, Day 1 |
≤ 60%, Day 2 |
||
B. arenarum |
|
Human pregnant urine |
None |
[2] |
B. baxteri |
4.0 ug LHRHa, Day 1 [no priming] |
500 IU, Day 1 |
None |
[35] |
0.8 ug LHRHa, Day −4 |
100 IU, Day −4 |
70%, Day 1-2 |
||
4.0 ug LHRHa, Day 1 |
500 IU, Day 1 |
|||
[total = 600 IU] | ||||
4.0 ug LHRHa, Day −6 |
500 IU, Day −6 |
80%, Day 1-2 |
||
0.8 ug LHRHa, Day −4 |
100 IU, Day −4 |
|||
4.0 ug LHRHa, Day 1 |
500 IU, Day 1 |
|||
[total = 1100 IU] | ||||
B. calamita |
|
Human pregnant urine |
None |
[2] |
B. fowleri |
|
Human pregnant urine |
+ |
[2] |
B. fowleri |
60 ug LHRHa, 5 mg progesterone, 0.25 mg pimozide, Day 1 |
500 IU, Day 1 |
85%, Day 1-2 |
[39] |
4 ug LHRHa, Day −1 |
500 IU, Day −1 |
29%, Day 1-2 |
||
4 ug LHRHa, Day 1 |
500 IU, Day 1 |
|||
[total = 1000 IU] | ||||
B. vulgaris |
|
Human pregnant urine |
None |
[2] |
Eleutherodactylus coqui |
|
25-140 IU, Day 1 |
None |
[40] |
165-200 IU, Day 1 |
Day 2, weak response (2/6 females) |
|||
Hyla aurea |
Pregnant mare serum |
- |
+ |
[2] |
Litoria aurea |
5 ug LHRHa, Day −4.5 |
300 IU, Day 1 |
Weak; 1% of normal egg release (2/5 females) |
[41] |
5 ug LHRHa, Day −1.5 | ||||
10 ug LHRHa, Day −0.5 | ||||
20 ug LHRHa, Day 1 | ||||
Litoria moorei |
No benefit of including progesterone |
100-200 IU, Day −2 |
Weak; few eggs in 1/14 females |
[41] |
500–750 IU, Day 1 | ||||
Litoria raniformis |
10 ug LHRHa, Day −4 |
500 IU, Day −4 |
None |
[42] |
10 ug LHRHa, Day −2 |
500 IU, Day −2 |
|||
10 ug LHRHa, Day 1 |
500 IU, Day 1 |
|||
Rana catesbeiana |
|
Human pregnant urine |
+ |
[2] |
R. catesbeiana |
Pregnant mare serum |
- |
+ |
[2] |
R. clamitans |
|
Human pregnant urine |
None |
[2] |
R. temporaria |
|
Human pregnant urine |
None |
[2] |
R. esculenta |
|
Human pregnant urine |
None |
[2] |
R. pipiens |
|
Human pregnant urine |
None |
[2] |
R. pipiens |
Pregnant mare serum |
- |
None |
[2] |
R. sevosa |
3-4 ug LHRH, Day −3 |
100 IU, Day −3 |
+ |
[37] |
15–20 ug LHRH, Day 1 |
500 IU, Day 1 |
|||
R. vulgaris |
|
Human pregnant urine |
None |
[2] |
Urodeles |
|
|
|
|
Ambystoma tigrinum |
|
Human pregnant urine |
+ |
[2] |
Triturus pyrrogaster |
|
Human pregnant urine |
+ |
[2] |
T. torosus; |
Pregnant mare serum |
- |
None |
[2] |
T. similans; | ||||
T. rivularis | ||||
T. viridescens | Human pregnant urine | + | [2] |
Results of attempts to induce oviposition utilising hCG administration in amphibian species other than Xenopus with, or without, priming with hCG or other agents. * Day of final hCG injection expressed as Day 1, with administration of compounds on days prior to Day 1 expressed as negative values (to facilitate comparison between studies listed in Tables 1 and 2); hCG administered as either reconstituted or synthetic hCG (dose in IU) or as direct injection of pregnant human urine (dose unknown). ** Oviposition: “+” = oviposition recorded; “None” = no oviposition recorded. Data from [2] for PMSG only inductions (no hCG) for some Rana species also shown. Species names are from original papers, however, some have since been renamed e.g. Rana pipiens now Lithobates pipiens by some [43].