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. 2012 Aug 21;10:60. doi: 10.1186/1477-7827-10-60

Table 2.

Results of attempts to induce oviposition in amphibians other thanXenopuswith hCG

Species Priming or co-administered agent hCG [dose or source]* Oviposition** References
Bufo americanus
 
Human pregnant urine
None
[2]
B. americanus
No priming
100 IU, Day 1
None
[38]
No priming
400-1000 IU, Day 1
about 60%, Day 2
Priming Day −2 [LH 50 ug, or LHRH 50 ug, or eCG 50 IU, or hCG 50 IU]
500 IU, Day 1
≤ 60%, Day 2
B. arenarum
 
Human pregnant urine
None
[2]
B. baxteri
4.0 ug LHRHa, Day 1 [no priming]
500 IU, Day 1
None
[35]
0.8 ug LHRHa, Day −4
100 IU, Day −4
70%, Day 1-2
4.0 ug LHRHa, Day 1
500 IU, Day 1
[total = 600 IU]
4.0 ug LHRHa, Day −6
500 IU, Day −6
80%, Day 1-2
0.8 ug LHRHa, Day −4
100 IU, Day −4
4.0 ug LHRHa, Day 1
500 IU, Day 1
[total = 1100 IU]
B. calamita
 
Human pregnant urine
None
[2]
B. fowleri
 
Human pregnant urine
+
[2]
B. fowleri
60 ug LHRHa, 5 mg progesterone, 0.25 mg pimozide, Day 1
500 IU, Day 1
85%, Day 1-2
[39]
4 ug LHRHa, Day −1
500 IU, Day −1
29%, Day 1-2
4 ug LHRHa, Day 1
500 IU, Day 1
[total = 1000 IU]
B. vulgaris
 
Human pregnant urine
None
[2]
Eleutherodactylus coqui
 
25-140 IU, Day 1
None
[40]
165-200 IU, Day 1
Day 2, weak response (2/6 females)
Hyla aurea
Pregnant mare serum
-
+
[2]
Litoria aurea
5 ug LHRHa, Day −4.5
300 IU, Day 1
Weak; 1% of normal egg release (2/5 females)
[41]
5 ug LHRHa, Day −1.5
10 ug LHRHa, Day −0.5
20 ug LHRHa, Day 1
Litoria moorei
No benefit of including progesterone
100-200 IU, Day −2
Weak; few eggs in 1/14 females
[41]
500–750 IU, Day 1
Litoria raniformis
10 ug LHRHa, Day −4
500 IU, Day −4
None
[42]
10 ug LHRHa, Day −2
500 IU, Day −2
10 ug LHRHa, Day 1
500 IU, Day 1
Rana catesbeiana
 
Human pregnant urine
+
[2]
R. catesbeiana
Pregnant mare serum
-
+
[2]
R. clamitans
 
Human pregnant urine
None
[2]
R. temporaria
 
Human pregnant urine
None
[2]
R. esculenta
 
Human pregnant urine
None
[2]
R. pipiens
 
Human pregnant urine
None
[2]
R. pipiens
Pregnant mare serum
-
None
[2]
R. sevosa
3-4 ug LHRH, Day −3
100 IU, Day −3
+
[37]
15–20 ug LHRH, Day 1
500 IU, Day 1
R. vulgaris
 
Human pregnant urine
None
[2]
Urodeles
 
 
 
 
Ambystoma tigrinum
 
Human pregnant urine
+
[2]
Triturus pyrrogaster
 
Human pregnant urine
+
[2]
T. torosus;
Pregnant mare serum
-
None
[2]
T. similans;
T. rivularis
T. viridescens   Human pregnant urine + [2]

Results of attempts to induce oviposition utilising hCG administration in amphibian species other than Xenopus with, or without, priming with hCG or other agents. * Day of final hCG injection expressed as Day 1, with administration of compounds on days prior to Day 1 expressed as negative values (to facilitate comparison between studies listed in Tables 1 and 2); hCG administered as either reconstituted or synthetic hCG (dose in IU) or as direct injection of pregnant human urine (dose unknown). ** Oviposition: “+” = oviposition recorded; “None” = no oviposition recorded. Data from [2] for PMSG only inductions (no hCG) for some Rana species also shown. Species names are from original papers, however, some have since been renamed e.g. Rana pipiens now Lithobates pipiens by some [43].